Figure 1. Typical EasySep™ Direct Human CTC Enrichment Profile
Starting with human whole blood from healthy donors, spiked with approximately 1% of CAMA cells (epithelial tumor cell line); the typical CTC (EpCAM+) content of non-lysed final enriched fraction is 79 ± 16 % (using the silver “Big Easy” EasySep™ Magnet; gated on DRAQ5™ for nucleated cells). Typically the log depletion of targeted CD45+ cells is 2.8 to 3.2. In the example above, CAMA cells were seeded into whole blood at a starting frequency of 0.98%. The CAMA cell (EpCAM+) content of the enriched fraction is 92.02% with a 3.8 log depletion of CD45+ cells.