STEMCELL Technologies EpiCult EpiCult-C Human Medium Kit
- 研究用
EpiCult™-C Medium(Human)(ST-05630)は、ヒト乳房管腔および筋上皮細胞の短期培養に最適化された無血清培地です。乳腺の管腔細胞、筋上皮細胞どちらの増殖もサポートします。
Collagenase/Hyaluronidase (製品コード:ST-07912)を添加した場合、ヒト乳房組織の酵素的解離にもご利用いただけます。
製品の特長
- ヒト乳腺上皮細胞のshort term cultureに最適化された無血清培地
- 乳腺の管腔細胞、筋上皮細胞どちらの増殖もサポート
- コラゲナーゼ/ヒアルロニダーゼを加えて、乳腺組織の解離に使用可能
- ウシ下垂体抽出物を含まず
乳腺上皮前駆細胞の分離・同定のワークフロー
Figure 1. Protocol for Isolation and Identification of Human and Mouse Mammary Epithelial Progenitor Cells
Phase contrast photographs of (A) a pure human myoepithelial cell colony, (B) a pure human luminal cell colony, and (C) a mixed human colony. (D) is a mouse colony. Unlike human mammary CFC colonies, subtypes of mouse mammary epithelial cell colonies are not easily identifiable. All colonies were cultured in either EpiCult™-B (Human: Catalog #05601) or EpiCult™-B (Mouse:Catalog #5610) in the presence of an irradiated NIH 3T3 feeder layer. Colonies were visualized by staining with Wright"s Giemsa. (E) is a picture of mammospheres obtained from primary human mammary epithelial cells and (F) is an image of tumorspheres obtained from MCF7 human breast cancer cell line.